Computational approaches are increasingly used to prioritize monoclonal antibody (mAB) candidates from BCR-seq datasets. However, the reliable identification of B cells encoding antibodies against rare antigenic epitopes remains challenging.
ORIGINAL PAPER
BCR repertoire analysis and cloning of antibody candidates targeting native and Asp7-isomerized β-amyloid
WHAT WE KNOW
We employed a repertoire-guided workflow combining antigen-tetramer sorting of B cells from immunized mice, followed by low-input bulk BCR-seq, which yielded informative clonal repertoires. Clustering analysis allowed us to prioritize IGH and IGK clonotypes potentially targeting {beta}-amyloid and the isoAsp7 proteoform (isoD7-A{beta}1-16), implicated in Alzheimer's disease. We observed recurrent IGHV8-12 and IGKV1-117 usage, consistent with canonical mouse anti-A{beta} responses.
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Review the primary source, validate the main result, and establish whether any listed-company transmission is direct.
EVIDENCE
What the evidence supports so far
Research signals
Classification pending.
What remains unverified
The full methodology, effect size, and limitations still require analyst review.
Company impact remains unverified until a direct economic transmission is established.